Detection of vitamins A and E by tandem mass spectrometry
AssigneeQuest Diagnostics Investments Incorporated
InventorsSum Chan, Qibo Jiang
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Abstract
A method for determining a combined amount of β-tocopherol and γ-tocopherol in a sample by tandem mass spectrometry includes subjecting the sample to ionization under conditions suitable to produce one or more ions from the β-tocopherol and γ-tocopherol detectable by mass spectrometry; and determining the amount of one or more of the ions by tandem mass spectrometry. Tandem mass spectrometry methods of this disclosure involve fragmenting β-tocopherol and γ-tocopherol ions having a mass to charge ratio of about 416.35±0.80 into one or more fragment ions including a fragment ion having a mass to charge ratio of about 151.00±0.80, wherein the amount of the one or more ions determined is related to the combined amount of β-tocopherol and γ-tocopherol in the sample.
Claims13
A kit for a quantitative assay of simultaneously detecting vitamin A and vitamin E in a sample, the kit comprising an internal standard for analyzing vitamin A and an internal standard for analyzing vitamin E using mass spectrometry.
Dependencies are inferred from the claim text.