Detection of symmetrical dimethylarginine
AssigneeIDEXX Laboratories, Inc.
InventorsHonghzhi Xie, Sreenivasa Rao Ramisetty, Murthy Vsn Yerramilli
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Abstract
The disclosure generally relates to the detection of symmetrical dimethylarginine (SDMA). More particularly, the disclosure relates to the detection of SDMA using a solid phase. The disclosure provides devices, reagents, kits and methods for detecting symmetrical dimethyl arginine (SDMA) in sample, such as a biological sample from an animal. The method includes detecting the presence or amount of SDMA in the sample by using an immunoassay format, such as a competitive immunoassay. The assay includes the use of antibodies to SDMA that are specific for SDMA and that have less affinity for other arginine derivatives.
Claims15
A device for determining symmetrical dimethyl arginine (SDMA) in a sample, comprising:
- a solid matrix comprising a particle non-diffusively bound thereto, wherein the particle comprises a capture reagent comprising an arginine derivative covalently attached to a protein, wherein the protein is non-covalently coated on the particle, wherein the arginine derivative is not SDMA, and wherein the arginine derivative is selected from the group consisting of asymmetrical dimethylarginine (ADMA), L-arginine, N-methylarginine (MMA), acylated ADMA, acylated L-arginine, and acylated MMA, and wherein the protein is selected from the group consisting of Bovine Serum Albumin (BSA), ovalbumin, Keyhole Limpet Hemocyanin (KLH), and Glucose-6-Phosphate Dehydrogenase (G6PDH).
Dependencies are inferred from the claim text.