Detection of mycobacterium on growth media

Abstract

To determine the presence of Mycobacterium in an environment, a sample from the environment can be plated onto a growth medium that is selective for Mycobacterium. Prior to plating, the sample may be treated with a compound containing sodium dodecyl sulfate containing glycine hydrochloride. Plating may occur immediately after the addition of the compound or after a standing period. The agar based growth medium can include a high concentration of crystal violet, in excess of 0.5 μg/ml. Mycobacterium colonies will generally appear white while other colonies will generally appear stained purple or another color.

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