Enhanced viral transduction of mammalian cells using material scaffolds
AssigneesNorth Carolina State University, The University of North Carolina at Chapel Hill
InventorsYevgeny Brudno, Pritha Agarwalla, Gianpietro Dotti
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Abstract
Disclosed are compositions and methods for a low cost, tunable, macroporous, alginate scaffold that transduces T cells with vectors under static condition.
Claims22
A method of transducing an immune cell, the method comprising a) obtaining a non-transduced immune cell and a viral vector encoding an exogenous gene, siRNA, tasiRNA, lncRNA, shRNA, mRNA, gRNA, or miRNA, and b) applying the non-transduced immune cell and the viral vector to a dry macroporous scaffold comprising pores sized to permit substantially homogenous distribution of immune cells throughout the scaffold, wherein application of the non-transduced immune cell and the viral vector to the dry macroporous scaffold results in conversion of the non-transduced immune cell to a transduced immune cell.
A method of making a chimeric antigen receptor (CAR) immune cell, the method comprising a) obtaining an immune cell that lacks a chimeric antigen receptor, b) applying the immune cell to a dry, macroporous scaffold comprising pores sized to permit substantially homogenous distribution of immune cells throughout the scaffold;
- wherein the scaffold comprises i) a ligand or antibody for a T cell receptor or NK cell receptor and ii) a viral vector encoding a chimeric antigen receptor, wherein application of the immune cell to the dry, macroporous scaffold results in conversion of the immune cell that lacks the chimeric antigen receptor to the chimeric antigen receptor immune cell.
Dependencies are inferred from the claim text.