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US 12,649,907 B2Granted
Alternate detergents for viral inactivation
AssigneesAmgen Inc., Avantor Performance Materials, Inc.
InventorsGabriella Perell, Rosa Daneshvar, Martina Kopp
Life of this patent
Estimating the term…
Abstract
Detergents for use to inactivate enveloped viruses, including detergents that are considered eco-friendly that can be used in biomanufacturing operations.
Claims23
A method for inactivating envelope virus in a fluid known or suspected to contain at least one enveloped virus, comprising
- obtaining a fluid known or suspected to contain at least one enveloped virus;
- exposing the fluid to a detergent having a CAS registry number of CAS 3055-99-0, CAS 9043-30-5, CAS 181135-58-0, CAS 181135-57-9, CAS 250692-65-0, CAS 228579-27-9, CAS 349477-49-2, CAS 70504-28-8, CAS 59080-45-4, CAS 69984-73-2, CAS 148616-91-5, CAS 148565-55-3, CAS 82494-09-5, CAS 253678-67-0, CAS 106402-05-5, or CAS 93911-12-7;
- at a detergent concentration that is 2.5× to 10× of its Critical Micelle Concentration (CMC) and time sufficient to cause viral inactivation.
A method for inactivating enveloped viruses during purification of a recombinant protein of interest comprising
- obtaining a fluid comprising the recombinant protein of interest which is known or suspected to contain at least one virus;
- subjecting the fluid to at least one detergent, wherein the detergent has a CAS registry number of CAS 3055-99-0, CAS 9043-30-5, CAS 181135-58-0, CAS 181135-57-9, CAS 250692-65-0, CAS 228579-27-9, CAS 349477-49-2, CAS 70504-28-8, CAS 59080-45-4, CAS 69984-73-2, CAS 148616-91-5, CAS 148565-55-3, CAS 82494-09-5, CAS 253678-67-0, CAS 106402-05-5, or CAS 93911 12-7 at a detergent concentration that is 5× of its Critical Micelle Concentration (CMC) and for at least 10 minutes to cause inactivation of enveloped viruses in the fluid;
- and
- subjecting the viral inactivated fluid to at least one unit operation which includes at least a filtration step or a chromatography step.
1 more element
A method for inactivating envelope virus in a fluid known or suspected to contain at least one enveloped virus, comprising
- obtaining a fluid known or suspected to contain at least one enveloped virus;
- exposing the fluid to a detergent which is TDA-9, at a detergent concentration that is 2.5X to 10X of its Critical Micelle Concentration (CMC) and time sufficient to cause viral inactivation.
Dependencies are inferred from the claim text.