High-expression type mesenchymal stem cells, culture method and use thereof

A method for culturing high-expression type mesenchymal stem cells, by co-culturing initial mesenchymal stem cells with specific proteins in a second medium for 24 to 196 h, wherein

• the initial mesenchymal stem cells can be procured from different sources including adipose tissue, dental pulp, bone marrow tissue and umbilical cord tissue of mammals;
• the second medium is a Neurobasal medium comprises 0.5-2 wt % of B-27 Supplement, 0.5-2 wt % of N-2 Supplement, 0.5-2 wt % of L-glutamine, 0.5-2 wt % of non-essential amino acids, 0.5-2 wt % of penicillin/streptomycin and 0.5-2 μg/mL of heparin;
• the specific proteins are at least one of Thrombospondin-1, Galectin-7, Alpha-2-HS-glycoprotein, Sparc protein, Keratin type II cytoskeletal 74, Nucleophosmin and FSTL1 protein;
• when the number of the initial mesenchymal stem cells is set as X1 and the concentration of the first specific protein in the medium is set as Y1 ng/ml, a ratio of X1 to Y1 is between 1×106:100 and 1×106:1,000;
• and
• the high-expression type mesenchymal stem cells can highly express LEFTY2 (Left-Right Determination Factor 2).