Polymers, methods of making polymers, and methods of coupling oligonucleotides to polymers
AssigneeIllumina, Inc.
InventorsBrian Mather, Weixian Xi
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Abstract
In some examples, a method of coupling oligonucleotides to a polymer is provided. Inactive moieties in a first region of a polymer may be selectively irradiated with light, while inactive moieties in a second region of the polymer are not irradiated, to generate first active moieties in the first region of the polymer. The first active moieties may be coupled to first oligonucleotides. The inactive moieties in the second region of the polymer may be irradiated with light to generate second active moieties in the second region of the polymer. The second active moieties may be coupled to second oligonucleotides.
Claims22
A method of coupling oligonucleotides to a polymer, the method comprising:
- selectively irradiating inactive moieties in a first region of a polymer with light, while not irradiating inactive moieties in a second region of the polymer, to generate first active moieties in the first region of the polymer;
- coupling the first active moieties to first oligonucleotides, wherein the first oligonucleotides comprise a mixture of first primers lacking an excision moiety and second primers comprising an excision moiety;
- irradiating the inactive moieties in the second region of the polymer with light to generate second active moieties in the second region of the polymer;
- and
- coupling the second active moieties to second oligonucleotides,
- wherein the first region of the polymer comprises a plurality of first subregions, and wherein the second region of the polymer comprises a plurality of second subregions.
A polymer, comprising a poly(cyclopropenone-masked dibenzocyclooctyne-acrylamide-co-acrylamide) copolymer having the structure:
A polymer, comprising a poly(dibenzocyclooctyne-acrylamide-co-acrylamide) copolymer having the structure:
Dependencies are inferred from the claim text.