Polypeptide variants and uses thereof
AssigneeGenmab B.V.
InventorsSimone Oostindie, Andreas Hollenstein, Frank Beurskens, Kristin Strumane, Janine Schuurman, Rob De Jong
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Abstract
The present invention relates to combination therapy involving two or more Fc region-containing antigen-binding polypeptides, such as antibodies, wherein the polypeptides have been modified such that hetero-oligomerization between the polypeptides is strongly favored over homo-oligomerization when the polypeptides are bound to their corresponding target antigens. The invention also relates to polypeptides, compositions, kits and devices suitable for use in the combination therapy of the invention.
Claims28
A polypeptide comprising a Fc region of a human IgG and an antigen-binding region capable of binding to an antigen, wherein said polypeptide comprises a Y436N, Y436K, Y436Q or Y436R mutation of an amino acid position corresponding to Y436 in human IgG1, and a K439F, K439I, K439Y, K439T, K439V, K439W, or K439E mutation of an amino acid position corresponding to K439 in human IgG1.
A polypeptide comprising a Fc region of a human IgG and an antigen-binding region capable of binding to an antigen, wherein said polypeptide comprises a Q438R, Q438H, Q438G or Q438N mutation of an amino acid position corresponding to Q438 in human IgG1, and an S440K mutation of an amino acid position corresponding to S440 in human IgG1.
A mixture of antibodies comprising:
- (a) a first antibody comprising an Fc region of a human IgG, wherein the Fc region comprises a Y436N, Y436K, Y436Q or Y436R mutation of an amino acid position corresponding to Y436 in human IgG1, and a K439F, K439I, K439Y, K439T, K439V, K439W, or K439E mutation of an amino acid position corresponding to K439 in human IgG1, and
- (b) a second antibody comprising an Fc region of a human IgG, wherein the Fc region comprises a Q438R, Q438K, Q438H, Q438G or Q438N mutation of an amino acid position corresponding to Q438 in human IgG1, and an S440K mutation of an amino acid position corresponding to S440 in human IgG1.
Dependencies are inferred from the claim text.