Antibody for tetanus toxin and use thereof
AssigneesBeijing Wisdomab Biotechnology Co., Ltd, Genrix (Shanghai) Biopharmaceutical Co. Ltd., Chongqing Genrix Biopharmaceutical Co., Ltd.
InventorsZhigang Liu, Xiaowei Zhou, Yulan Liu, Xiaobo Hao, Junjie Hu
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Abstract
Provided are a bispecific antibody and a monoclonal antibody for a tetanus toxin, and uses of the antibodies. The bispecific antibody contains a first antigen-binding fragment and a second antigen-binding fragment which bind to different epitopes of the tetanus toxin, and has activity of neutralizing the tetanus toxin. The monoclonal antibody binds to the tetanus toxin, and has activity of neutralizing the tetanus toxin.
Claims13
A bispecific antibody comprising a first antigen-binding fragment and a second antigen-binding fragment that bind to different epitopes of a tetanus toxin, wherein the bispecific antibody has activity of neutralizing the tetanus toxin,
- wherein the first antigen-binding fragment comprises:
- heavy chain complementarity determining region 1 (HCDR1) having the amino acid sequence of SYWIY (SEQ ID NO:
- 1),
- heavy chain complementarity determining region 2 (HCDR2) having the amino acid sequence of EINPTNGFANYNEKFKT (SEQ ID NO:
- 2) or EINPTAGFANYNEKFKT (SEQ ID NO:
- 3) or EINPTNAFANYNEKFKT (SEQ ID NO:
- 4),
- heavy chain complementarity determining region 3 (HCDR3) having the amino acid sequence of HFRFPY (SEQ ID NO:
- 5),
- light chain complementarity determining region 1 (LCDR1) having the amino acid sequence of RASQDIGSSLT (SEQ ID NO:
- 6),
- light chain complementarity determining region 2 (LCDR2) having the amino acid sequence of ATSSLDS (SEQ ID NO:
- 7), and
- light chain complementarity determining region 3 (LCDR3) having the amino acid sequence of LQYASSPYT (SEQ ID NO:
- 8);
- and
- wherein the second antigen-binding fragment comprises:
- HCDR1 having the amino acid sequence of DYGVN (SEQ ID NO:
- 9),
- HCDR2 having the amino acid sequence of MIWSDGTTDYSSALKS (SEQ ID NO:
- 10),
- HCDR3 having the amino acid sequence of VDGYSHYYAMDY (SEQ ID NO:
- 11),
- LCDR1 having the amino acid sequence of RASENIYSYLA (SEQ ID NO:
- 12),
- LCDR2 having the amino acid sequence of NAKTLAE (SEQ ID NO:
- 13), and
- LCDR3 having the amino acid sequence of QHHYGLPFT (SEQ ID NO:
- 14);
- wherein the HCDR and LCDR amino acid sequences are defined according to Kabat.
A monoclonal antibody that binds to a tetanus toxin, comprising:
- HCDR1 having the amino acid sequence of SYWIY (SEQ ID NO:
- 1),
- HCDR2 having the amino acid sequence of EINPTNGFANYNEKFKT (SEQ ID NO:
- 2) or EINPTAGFANYNEKFKT (SEQ ID NO:
- 3) or EINPTNAFANYNEKFKT (SEQ ID NO:
- 4),
- HCDR3 having the amino acid sequence of HFRFPY (SEQ ID NO:
- 5),
- LCDR1 having the amino acid sequence of RASQDIGSSLT (SEQ ID NO:
- 6),
- LCDR2 having the amino acid sequence of ATSSLDS (SEQ ID NO:
- 7), and
- LCDR3 having the amino acid sequence of LQYASSPYT (SEQ ID NO:
- 8);
- wherein the HCDR and LCDR amino acid sequences are defined according to Kabat.
A monoclonal antibody that binds to a tetanus toxin, comprising:
- HCDR1 having the amino acid sequence of DYGVN (SEQ ID NO:
- 9),
- HCDR2 having the amino acid sequence of MIWSDGTTDYSSALKS (SEQ ID NO:
- 10), HCDR3 having the amino acid sequence of VDGYSHYYAMDY (SEQ ID NO:
- 11), LCDR1 having the amino acid sequence of RASENIYSYLA (SEQ ID NO:
- 12),
- LCDR2 having the amino acid sequence of NAKTLAE (SEQ ID NO:
- 13), and
- LCDR3 having the amino acid sequence of QHHYGLPFT (SEQ ID NO:
- 14);
- wherein the HCDR and LCDR amino acid sequences are defined according to Kabat.
Dependencies are inferred from the claim text.