Scaffold proteins and therapeutic nanoconjugates based on nidogen

A conjugate comprising

• (A) a polypeptide scaffold comprising
• (i) a first polypeptide region comprising a variant of the G2 domain of nidogen-1
• corresponding to amino acid positions 430 to 667 of human nidogen-1 of SEQ ID NO:
• 72, and comprising mutations located at amino acid positions 459, 468, 639, and 650 of the nidogen-1 protein of SEQ ID NO:
• 72;
• (ii) a second polypeptide region comprising a polycationic peptide;
• (iii) a third polypeptide region comprising a positively charged amino acid-rich region;
• and,
• (B) an anticancer agent selected from the group consisting of antimetabolites and microtubule-disrupting agents, wherein the anticancer agent is conjugated to the polypeptide scaffold.