Isolated modified VP1 capsid protein of AAV5

An isolated altered VP1 protein of adeno-associated virus serotype 5 (AAV5) capsid for transduction of target cells, comprising the amino acid sequence of the VP1 protein of a wild-type AAV5 capsid, encoded by the Cap gene, comprising one or more substitutions selected from the group comprising:

• a S2A and T711S substitution, or
• a S2A, S651A and T711S substitution,
• wherein the amino acid sequence of the VP1 protein of a wild-type AAV5 capsid has the amino acid sequence of SEQ ID NO:
• 1.