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US 12,649,151 B2Granted
Manifolds, systems and methods for conducting biological studies under flow
Assignee3D Systems, Inc.
InventorsBagrat Grigoryan, Jordan Miller, Selene Alikah, Matthew Gelber, Faraz Jivan, Andres Ruiz, Jacob Snook
Life of this patent
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Abstract
Some embodiments of the disclosure disclose manifolds, microfluidic systems and methods that provide control over fluid flow distribution to an array of bio-scaffolds contained within the manifolds. In some embodiments, multiple perfusates may be injected into the manifold via multiple inlets where the manifold contains a bio-assembly with a substrate having a bio-scaffold disposed thereon. Biological investigations of the perfusates may then be conducted in the vascular components and chambers of the bio-scaffold.
Claims28
A manifold, comprising:
- a plate having one or more partitions, a first partition of the one or more partitions including:
- a first recess shaped and sized to receive a first bio-assembly having a first bio-assembly inlet, a first bio-assembly outlet and a first bio-scaffold that is disposed on a substrate,
- a bio-scaffold inlet of the first bio-scaffold being in fluid communication with the first bio-assembly inlet and a bio-scaffold outlet of the first bio-scaffold being in fluid communication with the first bio-assembly outlet;
- and
- an adhesive interface positioned within the first partition to interface with, and apply an adhesive to, the substrate of the first bio-assembly when the first bio-assembly is positioned in the first partition to attach the substrate of the first bio-assembly to the first partition;
- and
- a manifold inlet and a manifold outlet that are in fluid communication with the first bio-assembly inlet and the first bio-assembly outlet, respectively, when the first bio-assembly is positioned in the first partition;
- wherein the first partition further includes a bio-scaffold access channel coupled to a second bio-assembly outlet of the first bio-assembly and configured to transport fluids between an interior of the first bio-assembly and outside the first bio-assembly via the second bio-assembly outlet.
5 more elements
A manifold, comprising:
- a plate having one or more partitions, a first partition of the one or more partitions including:
- a first recess shaped and sized to receive a first bio-assembly having a first bio-assembly inlet, a first bio-assembly outlet and a first bio-scaffold that is disposed on a substrate,
- a bio-scaffold inlet of the first bio-scaffold being in fluid communication with the first bio-assembly inlet and a bio-scaffold outlet of the first bio-scaffold being in fluid communication with the first bio-assembly outlet;
- and
- an adhesive interface positioned within the first partition to interface with, and apply an adhesive to, the substrate of the first bio-assembly when the first bio-assembly is positioned in the first partition to attach the substrate of the first bio-assembly to the first partition;
- and
- a manifold inlet and a manifold outlet that are in fluid communication with the first bio-assembly inlet and the first bio-assembly outlet, respectively, when the first bio-assembly is positioned in the first partition;
- wherein the adhesive interface includes a moat configured to prevent fluid leakage from the first partition;
- wherein the moat contains ultra-violet or visible light curable resin, air, cyanoacrylate adhesive, silicone gasket, or any combination thereof.
6 more elements
A manifold, comprising:
- a plate having one or more partitions, a first partition of the one or more partitions including:
- a first recess shaped and sized to receive a first bio-assembly having a first bio-assembly inlet, a first bio-assembly outlet and a first bio-scaffold that is disposed on a substrate,
- a bio-scaffold inlet of the first bio-scaffold being in fluid communication with the first bio-assembly inlet and a bio-scaffold outlet of the first bio-scaffold being in fluid communication with the first bio-assembly outlet;
- and
- an adhesive interface positioned within the first partition to interface with, and apply an adhesive to, the substrate of the first bio-assembly when the first bio-assembly is positioned in the first partition to attach the substrate of the first bio-assembly to the first partition;
- and
- a manifold inlet and a manifold outlet that are in fluid communication with the first bio-assembly inlet and the first bio-assembly outlet, respectively, when the first bio-assembly is positioned in the first partition;
- wherein the first bio-scaffold includes a surface having a sectioning label covalently photocrosslinked thereon and configured to facilitate removal of the first bio-scaffold from the first bio-assembly after the first bio-assembly is positioned in the first partition and the substrate of the first bio-assembly is attached to the first partition.
5 more elements
Dependencies are inferred from the claim text.