Enzyme-decorated nanocatalysts
AssigneeCassiopea S.P.A.
InventorsKeith Laderoute, Mark P. Andrews, Egor Katkov, Sally Royal Hamry, Janine Mauzeroll, John Thomas Marino
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Abstract
Provided herein are vesicular nanocatalysts decorated on their surface with enzymes, such as for industrial use. More particularly, the nanocatalysts include outer membrane vesicles (OMVs) presenting on their surface carbonic anhydrase or a functional fragment or derivative thereof. Uses, methods of manufacture, and compositions of the nanocatalysts are also disclosed herein.
Claims18
An engineered outer membrane vesicle (OMV) comprising a carbonic anhydrase (CA) polypeptide presented on the outer surface of the engineered OMV, wherein the engineered OMV is produced from engineered cyanobacteria Synchronous sp. comprising:
- a fusion protein comprising the CA polypeptide having the amino acid sequence of SEQ ID NO:
- 7 operably linked to a transmembrane protein having the amino acid sequence of any one of SEQ ID NOs:
- 8-12.
An engineered host cell capable of producing an engineered outer membrane vesicle (OMV) comprising a carbonic anhydrase (CA) polypeptide presented on the outer surface of the OMV, wherein the engineered host cell is an engineered photosynthetic microorganism Synchronous sp. comprising a heterologous nucleic acid encoding a fusion protein, wherein the fusion protein comprises the CA polypeptide having the amino acid sequence of SEQ ID NO:
- 7 operably linked to a transmembrane protein having the amino acid sequence of any one of SEQ ID Nos:
- 8-12, and wherein the fusion protein is capable of being inserted into the outer membrane of the engineered host cell such that CA polypeptide is presented on the outer surface of the engineered host cell.
Dependencies are inferred from the claim text.