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US 12,649,116 B2Granted
Integrated dual-mode chromatography to enrich extracellular vesicles from plasma
AssigneeThe General Hospital Corporation
InventorsHakho Lee, Ralph Weissleder, Jan Van Deun
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Abstract
The present disclosure provides, in some embodiments, devices, methods, and kits for purifying extracellular vesicles (EVs) using size exclusion chromatography in tandem with cation exchange chromatography, which can be referred to as dual-mode chromatography (DMC).
Claims18
A method for purifying extracellular vesicles (EVs) from a sample comprising EVs, high density lipoprotein (HDL), and lipoprotein particles (LPPs), the method comprising:
- contacting the sample comprising the EVs with a size exclusion resin for a time and under conditions sufficient to separate HDL from the EVs;
- contacting the sample with a cation exchange resin for a time and under conditions sufficient to bind LPPs;
- and
- collecting flow-through comprising the EVs, wherein the flow-through is substantially depleted of HDL and LPPs.
1 more element
Dependencies are inferred from the claim text.