Recombinant BCG, preparation method therefor and application thereof
AssigneeXuzhou Hairun Biotechnology Co., Ltd.
InventorsYang Zhang, Pengjv Li
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Abstract
Recombinant BCG, a preparation method therefor and an application thereof. A shuttle plasmid that can express a FimH protein on the surface of BCG is constructed, and a gene fragment that expresses the FimH protein is transformed into wild-type BCG to thereby obtain recombinant BCG. Upon verification, the recombinant BCG can express the FimH protein on the surface of BCG; therefore, the BCG can specifically bind to subjects that can selectively bind to the FimH protein. The recombinant BCG can also enhance the local innate immune effect and adaptive immune effect induced by BCG and the anti-tumor effect of peripheral blood mononuclear cells, and also significantly improves the effect of BCG against bladder tumors, and is used to treat bladder tumors.
Claims4
A recombinant Bacille Calmette-Guérin (BCG), wherein the recombinant BCG is a BCG capable of expressing a FimH protein on its own surface, wherein the recombinant BCG is deposited at China General Microbiological Culture Collection Center with an accession number of 19540, and wherein the recombinant BCG, via the FimH protein expressed on its surface, specifically binds to subjects with FimH protein-binding properties.
Dependencies are inferred from the claim text.