Humanized chimeras for the prospective assessment of cell addition and replacement therapies
AssigneesUniversity of Rochester, University of Copenhagen
InventorsSteven A. Goldman, Ricardo da Costa Barbedo Vieira
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Abstract
A chimeric non-human mammal disease model, wherein (1) at least 30% of all the glial cells in the corpus callosum of the chimeric non-human mammal are human glial cells, and/or (2) at least 5% of all of the glial cells in the white matter of the brain and/or brain stem of the chimeric non-human mammal are human glial cells, and wherein the human glial cells comprise a combination of a first group of human glial cells tagged with a first label and a second group of human glial cells tagged with a second label that is distinguishable from the first label.
Claims18
A method for producing a chimeric non-human mammal comprising human glial cells, the method comprises the steps of:
- firstly introducing a first population of human glial progenitor cells into the brain and/or brain stem of a non-human mammal, wherein the first population of human glial progenitor cells are tagged with a first detectable label;
- secondly introducing a second population of human glial progenitor cells into the brain and/or brain stem of the non-human mammal, wherein the second population of human glial progenitor cells are tagged with a second detectable label that is distinguishable from the first detectable label;
- thirdly recovering, as a result of said introducing, the chimeric non-human mammal with human glial cells comprising combination of first human glial cells that are (1) derived or differentiated from the first population of human glial progenitor cells and (2) tagged with the first detectable label, and second human glial cells that are (1) derived or differentiated from the second population of human progenitor cells and (2) tagged with the second detectable label,
- wherein (1) at least 30% of all the glial cells in the corpus callosum of the chimeric non-human mammal are human glial cells, and/or (2) at least 5% of all of the glial cells in the white matter of the brain and/or brain stem of the chimeric non-human mammal are human glial cells, and
- wherein at least some of the second human glial cells induce apoptosis of some of the first human glial cells.
Dependencies are inferred from the claim text.