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AssigneeKyushu University, National University Corporation
InventorsTakahiro Nakamura, Yusuke Yagi, Keiko Kobayashi
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A method for designing a protein capable of binding in an RNA base selective manner or RNA base sequence specific manner is provided. The protein of the present invention is a protein containing one or more of PPR motifs (preferably 2 to 14 PPR motifs) each consisting of a polypeptide of 30- to 38-amino acid length represented by the formula 1 (wherein Helix A is a moiety of 12-amino acid length capable of forming an α-helix structure, and is represented by the formula 2, wherein, in the formula 2, A1 to A12 independently represent an amino acid; X does not exist, or is a moiety of 1- to 9-amino acid length; Helix B is a moiety of 11- to 13-amino acid length capable of forming an α-helix structure; and L is a moiety of 2- to 7-amino acid length represented by the formula 3, wherein, in the formula 3, the amino acids are numbered “i” (−1), “ii” (−2), and so on from the C-terminus side, provided that Liii to Lvii may not exist), and combination of three amino acids A1, A4 and Lii, or combination of two amino acids A4, and Lii is a combination corresponding to a target RNA base or base sequence.
A method for modifying or disrupting an RNA having a target sequence, or imparting a novel function to an RNA having a target sequence, in a living body or cell, the method comprising:
A method for modifying or disrupting an RNA having a target sequence, or imparting a novel function to an RNA having a target sequence, in a living body or cell, the method comprising:
A method for preparing a polynucleotide consisting of a nucleic acid sequence coding for an amino acid sequence of a complex comprising a region consisting of a PPR protein and a functional region, the PPR protein being able to bind to an RNA having a target sequence, the method comprising:
Dependencies are inferred from the claim text.