Large animal model of traumatic optic neuropathy

A method for constructing a clinically relevant large animal model of traumatic optic neuropathy, comprising:

• (1) preoperatively selecting bodies of sphenoid bones of goat skulls using CT scanning and confirming a surgical pathway:
• performing skull CT plain scan and three-dimensional model reconstruction for a plurality of goats before surgery, and selecting goats meeting the following conditions as goat models of quantitative injury:
• a. a maximum transverse diameter of a coronal plane of a body of sphenoid bone of the selected goat is 14-16 mm;
• b. pneumatization of a body cavity of the sphenoid bone is well;
• and
• c. the sphenoid bone has a wall width of less than 2 mm;
• (2) preoperatively anesthetizing and preparing:
• anesthetizing the selected goats by intramuscular injection of xylazine, intubating with endotracheal tubes, and maintaining the selected goats under anesthesia with 3.5% isoflurane and a 50/50% air-oxygen mixture at a constant flow rate of 2 L/min, with the selected goats covered with blankets to maintain core body temperature during anesthesia and recovery;
• and
• (3) preparing the goat model of traumatic optic neuropathy:
• making an incision in skin of a nasal cavity, suspending the skin to both sides, grinding bones under the skin to expose the nasal cavity, removing a middle turbinate, finding an upper frontal sinus and straightly cutting soft tissues below the upper frontal sinus, clearing an olfactory nerve to expose a white body of sphenoid bone, making an incision at a connection part of a nasal septum and the body of sphenoid bone using a sickle knife, then extending the incision upwards and downwards using a curved scissor to free and remove the nasal septum, exposing and straightly cutting the left olfactory nerve, removing bones at the connection part of the nasal septum and the body of sphenoid bone using a rongeur to expose a bone wall in front of a sphenoid sinus, removing the bone wall in front of the sphenoid sinus by dynamic rough grinding, and removing contents in the sphenoid sinus to expose a bone wall of an optic canal;
• directly striking the bone wall of the optic canal with a striking device to obtain a quantitative injury model, rinsing the nasal cavity with iodophor, and then placing a gelatin sponge;
• checking that there is no residual foreign body in each cavity, and stopping anesthesia;
• and suturing the skin and periosteum using splay suture and interrupted suture to close the nose to obtain the large animal model of traumatic optic neuropathy.