Information processing device, biological sample observation system, and image generation method

A biological sample observation system, comprising:

• an imaging device that acquires a non-stained image including an autofluorescence component;
• and
• an information processing device that processes the non-stained image, wherein
• the information processing device includes
• a simulated image generation unit that generates a simulated image by superimposing the non-stained image and a dye tile image in which a reference spectrum of a first fluorescent dye and imaging noise of each of pixels of the non-stained image are associated,
• a fluorescence separation unit that separates a component of the first fluorescent dye and the autofluorescence component on the basis of the simulated image and generates a separated image, and
• an evaluation unit that evaluates a degree of separation of the separated image.

An image generation method, comprising

• generating a simulated image by superimposing a non-stained image including an autofluorescence component and a dye tile image in which a reference spectrum of a first fluorescent dye and imaging noise of each of pixels of the non-stained image are associated;
• separating a component of the first fluorescent dye and the autofluorescence component on the basis of the simulated image and generates a separated image;
• and
• evaluating a degree of separation of the separated image.