Back to results
US 12,644,890 B2Granted
Methods and devices from isolation of tumor cells
AssigneeElios Holdings, LLC
InventorsThomas E. Wagner, Amanda J. Sloan, Xianzhong Yu
Life of this patent
Estimating the term…
Abstract
Described herein are methods and compositions for isolating cancer cells using positively charged surfaces, such as beads, microparticles, and capillary tubes. The methods and compositions for isolation can be used in cancer diagnosis, treatment, and therapeutic preparation.
Claims18
A method of removing cancer cells from a biological sample, comprising:
- a) passing a biological sample comprising cancer cells obtained from a subject over an isolation matrix comprising a positively charged surface, wherein the positively charged surface comprises glass that is functionalized with a positively charged functional group selected from the group consisting of an amine, an aldehyde, polyethylenimine (PEI), a guanidine group, and a combination thereof;
- and
- b) collecting the biological sample that flows through the isolation matrix comprising the positively charged surface in a first aliquot,
- wherein the cancer cells bind to the isolation matrix.
1 more element
A method of detecting a cancer cell in a patient, comprising:
- a) passing a biological sample from the patient through a functionalized glass capillary tube or an isolation matrix of functionalized glass microspheres, wherein the functionalized glass capillary tube or the functionalized glass microspheres comprise amine, polyethylenimine (PEI), and/or guanidine groups that are attached to the glass capillary tube or glass microsphere via a siloxane bond, and wherein the functionalized glass capillary tube or the functionalized glass microspheres bind cancer cells;
- b) eluting the biological sample that flows through the functionalized glass capillary tube or the isolation matrix;
- and
- c) detecting the presence of a cancer cell bound to the functionalized glass capillary tube or functionalized glass microspheres.
1 more element
A method of binding a cancer cell from a biological sample, comprising:
- a) contacting a biological sample comprising a cancer cell with an isolation matrix comprising glass beads having surfaces that are functionalized with a positively charged functional group which may be any one or more of an amine, an aldehyde, polyethyleneimine (PEI), or a guanidine group;
- b) washing the isolation matrix to remove the biological sample from the isolation matrix, wherein the cancer cell binds to the isolation matrix;
- c) lysing the cancer cell, thereby providing a lysate;
- and
- d) detecting in the lysate one or more nucleic acids.
2 more elements
Dependencies are inferred from the claim text.