Back to results
US 12,644,835 B2Granted
Continuous scanning optical assembly and method of use thereof
AssigneeTruvian Sciences, Inc.
InventorsRoger Taylor, Michael Shane Bowen, George Brabon, Michael Adams, Jeff Hawkins
Life of this patent
Estimating the term…
Abstract
The present disclosure provides an automated sample analyzer having a continuous scanning optical assembly for performing an assay. The optical assembly allows for robust detection of light emitted from a reaction mixture in a dynamically changing environment, such as detection of light from a reaction mixture that is being rotated about an axis at high rotational velocity.
Claims17
An automated sample analyzer comprising:
- a) a rotation assembly operable to start and stop continuous rotation of a substrate, the substrate having a well disposed within a perimeter of the substrate, wherein the well is configured to hold a reaction mixture including a sample and reagent wherein the reaction mixture comprises a fluorescently labeled moiety, wherein the fluorescently labeled moiety comprises a bead and wherein the bead is magnetic;
- b) an optical assembly having an illumination source and an illumination detector, wherein the optical assembly is operable to irradiate the reaction mixture with light emitted from the illumination source and detect emission light from the reaction mixture via the illumination detector, and
- wherein the optical assembly is configured to generate a coincidence focal point comprising an illumination light path focal point and a detection light path focal point on a plane of rotation of the substrate;
- and
- c) a processor operable to control movement of the rotation assembly, wherein the processor includes functionality to cause the rotation assembly to continually rotate the substrate and adjust a height of the plane of rotation of the substrate relative to the coincidence focal point.
2 more elements
An automated sample analyzer comprising:
- a) a rotation assembly operable to start and stop continuous rotation of a substrate, the substrate having a well disposed within a perimeter of the substrate, wherein the well is configured to hold a reaction mixture including a sample and reagent further comprising a magnetic source configured to interact with the reaction mixture;
- b) an optical assembly having an illumination source and an illumination detector, wherein the optical assembly is operable to irradiate the reaction mixture with light emitted from the illumination source and detect emission light from the reaction mixture via the illumination detector, and
- wherein the optical assembly is configured to generate a common illumination light path focal point and detection light path focal point on a plane of rotation of the substrate;
- and
- c) a processor operable to control movement of the rotation assembly, wherein the processor includes functionality to cause the rotation assembly to continually rotate the substrate and adjust a height of the plane of rotation of the substrate relative to the common focal points.
2 more elements
An automated sample analyzer comprising:
- a) a rotation assembly operable to start and stop continuous rotation of a substrate, the substrate having a well disposed within a perimeter of the substrate, wherein the well is configured to hold a reaction mixture including a sample and reagent;
- b) an optical assembly having an illumination source and an illumination detector, wherein the optical assembly is operable to irradiate the reaction mixture with light emitted from the illumination source and detect emission light from the reaction mixture via the illumination detector, wherein the illumination source light path includes an objective lens, wherein the optical assembly is configured such that a back aperture of the lens is under filled and
- wherein the optical assembly is configured to generate a common illumination light path focal point and detection light path focal point on a plane of rotation of the substrate;
- and
- c) a processor operable to control movement of the rotation assembly, wherein the processor includes functionality to cause the rotation assembly to continually rotate the substrate and adjust a height of the plane of rotation of the substrate relative to the common focal point.
2 more elements
Dependencies are inferred from the claim text.