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US 12,644,827 B2Granted
Systems and methods for panel design in flow cytometry
AssigneeBeckman Coulter, Inc.
InventorMichael Kapinsky
Life of this patent
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Abstract
Embodiments of the present invention encompass systems and methods for determining detection limits for various antibody-dye conjugates for flow cytometry. Exemplary techniques involve a linear superpositioning approach of spillover-induced enlargements of normally distributed measurement errors.
Claims20
A method of designing a probe panel for a flow cytometer, the method comprising:
- identifying a first probe, a second probe, and a third probe;
- identifying a plurality of possible probe panels, each possible probe panel including a combination of the first probe, the second probe, or the third probe, each probe having a possible label associated thereto:
- evaluating a first possible probe panel by determining a detection limit of the first probe based on spectrum spillover effects of combination of the second probe and its associated possible label;
- evaluating a second possible probe panel by determining a detection limit of the second probe based on spectrum spillover effects of the combination of the third probe and its associated possible label;
- and
- selecting the probe panel from the plurality of possible probe panels based on the detection limits determined.
3 more elements
A method of designing a probe panel for a flow cytometer, the method comprising:
- identifying a first probe, a second probe, and a third probe;
- identifying a plurality of possible probe panels, each possible probe panel including a combination of the first probe, the second probe, or the third probe, each probe having a possible label associated thereto:
- evaluating a first possible probe panel by determining a detection limit of the first probe based on spectrum spillover effects of combination of the second probe and its associated possible label;
- evaluating a second possible probe panel by determining a detection limit of the second probe based on spectrum spillover effects of the combination of the third probe and its associated possible label;
- selecting the probe panel from the plurality of possible probe panels based on the detection limits determined;
- and
- displaying a graphical representation of a population distribution of expected signals for a pair of probes in the selected probe panel,
- wherein evaluating the first possible probe panel includes determining the detection limit of the first probe based in part on a coexpression pattern of antigens associated with the first probe and the second probe.
5 more elements
A method of designing a probe panel for a flow cytometer, the method comprising:
- identifying a first probe, a second probe, and a third probe;
- identifying a plurality of possible probe panels, each possible probe panel including a combination of the first probe, the second probe, or the third probe, each probe having a possible label associated thereto:
- evaluating a first possible probe panel by determining a detection limit of the first probe based on spectrum spillover effects of combination of the second probe and its associated possible label;
- evaluating a second possible probe panel by determining a detection limit of the second probe based on spectrum spillover effects of the combination of the third probe and its associated possible label;
- selecting the probe panel from the plurality of possible probe panels based on the detection limits determined;
- and
- displaying a graphical representation of a population distribution of expected signals for a pair of probes in the selected probe panel,
- wherein evaluating the first possible probe panel includes determining the detection limit of the first probe based in part on a coexpression pattern of antigens associated with the first probe and the second probe,
- wherein the coexpression pattern of antigens further comprises coexpression relationships between antigens for a particular cell type.
6 more elements
Dependencies are inferred from the claim text.