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US 12,644,203 B2Granted
Cellular phenotype screening method
AssigneesThinkCyte K.K., The University of Tokyo, Riken
InventorsAsako Tsubouchi, Sadao Ota, Fumiko Kawasaki
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Abstract
The present disclosure provides a method for screening cells, the method including a step of preparing a plurality of cells which are tagged with a first barcode nucleic acid associated with a test target and treated with the test target, a step of sorting the plurality of cells based on cellular phenotype using an imaging cell sorter, and a step of identifying the test target used to treat each cell using the first barcode nucleic acid as an indicator.
Claims12
A method for screening a target position causing a phenotype change to a cell, the method comprising:
- preparing a plurality of cells which are tagged with a first barcode nucleic acid corresponding to information identifying the location where gene editing occurs or information on guide RNA used for gene editing;
- sorting the plurality of cells based on cellular phenotype using an imaging cell sorter;
- and
- identifying the target position using the first barcode nucleic acid as an indicator,
- wherein the first barcode nucleic acid includes an anchor able to link the first barcode nucleic acid to a cell surface.
2 more elements
Dependencies are inferred from the claim text.