Sorghum cytoplasmic male sterility markers and loci
AssigneePioneer Hi-Bred International, Inc.
InventorsSunita R Chilakamarri, David Alan Hessel, Siva S Ammiraju Jetty, Maria Laura Mayor
Life of this patent
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Abstract
Various methods and compositions are provided for identifying and/or selecting a sorghum plant or germplasm with or without a cytoplasmic male sterility (CMS) trait. In certain embodiments, the method comprises detecting at least one allele of one or more marker locus within or linked to a QTL associated with CMS. In further embodiments, the method comprises crossing a selected sorghum plant with a recurrent sorghum parent plant and selecting progeny with CMS.
Claims3
A method of selecting a sorghum plant or sorghum germplasm with cytoplasmic male sterility (CMS), the method comprising:
- (a) detecting in tissue from a sorghum plant or sorghum germplasm, one or more markers linked to a quantitative trait locus (QTL) associated with CMS, wherein the one or more markers comprises:
- (1) SEQ ID NO:55 having the C allele at position 32084, wherein the position is relative to SEQ ID NO:
- 271;
- (2) SEQ ID NO:59 having the T allele at position 72950, wherein the position is relative to SEQ ID NO:
- 271;
- (3) SEQ ID NO:61 having the C allele at position 315577, wherein the position is relative to SEQ ID NO:
- 271;
- (4) SEQ ID NO:62 having the A allele at position 347518, wherein the position is relative to SEQ ID NO:
- 271;
- or
- (5) SEQ ID NO:63 having the A allele at position 373170, wherein the position is relative to SEQ ID NO:
- 271;
- or combinations thereof;
- and
- (b) selecting the sorghum plant or sorghum germplasm, comprising the one or more markers linked to the QTL associated with CMS detected in step (a), thereby selecting the sorghum plant or sorghum germplasm with CMS.
A method of introgressing a sorghum plant with cytoplasmic male sterility (CMS), the method comprising:
- (a) crossing a sorghum plant having CMS with a sorghum plant not having CMS to create a population of progeny sorghum plants or sorghum germplasm;
- (b) detecting in tissues from the population of progeny sorghum plants or sorghum germplasm from step (a) a marker linked to a quantitative trait locus (QTL) associated with CMS, wherein the one or more markers comprises:
- (1) marker SEQ ID NO:55 having the C allele at position 32084, wherein the position is relative to SEQ ID NO:
- 271;
- (2) marker SEQ ID NO:59 having the T allele at position 72950, wherein the position is relative to SEQ ID NO:
- 271;
- (3) marker SEQ ID NO:61 having the C allele at position 315577, wherein the position is relative to SEQ ID NO:
- 271;
- (4) marker SEQ ID NO:62 having the A allele at position 347518, wherein the position is relative to SEQ ID NO:
- 271;
- or
- (5) marker SEQ ID NO:63 having the A allele at position 373170, wherein the position is relative to SEQ ID NO:
- 271, or combinations thereof;
- (c) from the population of progeny sorghum plants or sorghum germplasm, selecting one or more progeny sorghum plants or sorghum germplasm comprising the marker linked to the QTL associated with CMS detected in step (b), thereby selecting one or more sorghum plants or sorghum germplasm with CMS.
A method of hybrid sorghum seed production comprising:
- (a) detecting in tissue from a sorghum plant or sorghum germplasm, one or more markers linked to a quantitative trait locus (QTL) associated with CMS, wherein the one or more markers comprises:
- (1) marker SEQ ID NO:55 having the C allele at position 32084, wherein the position is relative to SEQ ID NO:
- 271;
- (2) marker SEQ ID NO:59 having the T allele at position 72950, wherein the position is relative to SEQ ID NO:
- 271;
- (3) marker SEQ ID NO:61 having the C allele at position 315577, wherein the position is relative to SEQ ID NO:
- 271;
- (4) marker SEQ ID NO:62 having the A allele at position 347518, wherein the position is relative to SEQ ID NO:
- 271;
- or
- (5) marker SEQ ID NO:63 having the A allele at position 373170, wherein the position is relative to SEQ ID NO:
- 271, or combinations thereof;
- and
- (b) selecting the sorghum plant or sorghum germplasm comprising the marker linked to the QTL associated with CMS detected in step (a), thereby selecting the sorghum plant or sorghum germplasm with CMS;
- (c) planting the sorghum plant or sorghum germplasm selected in step (b) in rows alternating with sorghum plants or sorghum germplasm without CMS;
- (d) fertilizing the planted sorghum plants or sorghum germplasm that have CMS with pollen from sorghum plants that do not have CMS;
- and
- (e) harvesting seeds from the sorghum plants or sorghum germplasm fertilized in step (d).
Dependencies are inferred from the claim text.