Device and methods for detecting fungal pathogenicity in postharvest produce

A system for detecting fungi in a plant tissue, said system comprises:

• a. a measuring transducer;
• b. a surface;
• and
• c. nucleic acid strands immobilized onto said surface;
• wherein said system comes in contact with a mixture comprising:
• (i) target RNA sequences from said fungi;
• and
• (ii) reporter strands conjugated to a signal-generating component;
• wherein said target RNA sequences from said fungi anneal to said nucleic acid strands immobilized onto said surface of said system, further wherein said reporter strands anneal to said target RNA sequences, further wherein said signal-generating component is configured to generate a detectable reaction, further wherein said system is configured to indicate the developmental stage of said fungi,
• further wherein said nucleic acid strands immobilized onto said surface comprise SEQ ID NO 2.