Methods and devices for detecting and identifying microorganisms

A method for depleting eukaryotic DNA from a sample from a subject comprising adding a eukaryotic cell lysis solution to the sample, wherein the eukaryotic cell lysis solution selectively targets and predominantly lyses eukaryotic cells as opposed to microbial cells, wherein the eukaryotic cell lysis solution combined with the sample contains less than about 0.15 M of monovalent salts and comprises between 0.25% to 1% (v/v) of polysorbate 20, polysorbate 40, polysorbate 80, or any combination thereof, and between 0.2% to 0.65% (v/v) of octoxynol-9, octoxynol-8, nonoxynol-5, nonoxynol-9, nonoxynol-12, or any combination thereof.