Molecular probe for nucleic acid detection, preparation and use thereof
AssigneeGe Healthcare Limited
InventorsZhoushe Zhao, Hongli Li, Yanmei Wang
Life of this patent
Estimating the term…
Abstract
The present disclosure relates to molecular probes for nucleic acid detection, and preparation and uses thereof. In particular, described herein is a molecular probe for detection of a nucleic acid, comprising, (1) a molecular probe carrier which comprises a cell penetrating peptide and a detectable label coupled to the cell penetrating peptide, and (2) a targeting oligonucleotide, wherein the targeting oligonucleotide is attached to the molecular probe carrier. Also described herein are a method for preparing the molecular probe, a method for detection of a nucleic acid, and a kit for detection of a nucleic acid.
Claims8
A molecular probe for detection of a nucleic acid, comprising,
- (1) a molecular probe carrier comprising
- a cell penetrating peptide wherein the cell penetrating peptide comprises an amino acid sequence having 100% sequence identity with the amino acid sequence set forth in SEQ ID NO:
- 1;
- and a detectable label coupled to the cell penetrating peptide wherein the detectable label is coupled to a lysine residue comprised in the cell penetrating peptide, and
- (2) a targeting oligonucleotide, wherein the targeting oligonucleotide is attached to the molecular probe carrier.
A method for preparing a molecular probe, comprising,
- coupling a detectable label to a cell penetrating peptide to obtain a molecular probe carrier, wherein the cell penetrating peptide comprises an amino acid sequence having 100% sequence identity with the amino acid sequence set forth in SEQ ID NO:
- 1 and the detectable label is coupled to a lysine residue comprised in the cell penetrating peptide;
- incubating the molecular probe carrier with a targeting oligonucleotide for a duration of time sufficient to allow attachment between the molecular probe carrier and the targeting oligonucleotide, to obtain the molecular probe.
Dependencies are inferred from the claim text.