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US 12,644,141 B2Granted
Enzymatic modification of oil
AssigneeDSM Nutritional Products AG
InventorsJaroslav A. Kralovec, Weijie Wang, James Colin Barrow
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Abstract
The disclosed subject matter relates generally to a method for modifying oil, and specifically to a process for increasing the concentration of polyunsaturated fatty acid in an oil composition. The process comprises a) hydrolysis of the glyceride by Thermomyces lanuginosus lipase, b) separation of the free saturated fatty acids from the glycerides and c) enzymatic esterification of the hydrolyzed glyceride by Candida antarctica lipase B with a polyunsaturated fatty acid.
Claims19
A method for modifying an oil comprising a glyceride, comprising:
- a) contacting the oil with an aqueous solution of Thermomyces lanuginosus lipase at a time and temperature sufficient to hydrolyze at least a portion of the glyceride and thereby provide a composition comprising a free saturated fatty acid fraction and a hydrolyzed glyceride fraction;
- b) separating at least a portion of the free saturated fatty acid fraction from the hydrolyzed glyceride fraction;
- and
- c) esterifying, in the presence of a second lipase, the hydrolyzed glyceride fraction from step b) with a polyunsaturated fatty acid or ethyl ester thereof to form a re-esterified oil comprising greater than or equal to 60%, by weight, triglycerides and greater than or equal to 50%, by weight, omega-3 polyunsaturated fatty acids based on a total weight of the re-esterified oil.
1 more element
Dependencies are inferred from the claim text.