Back to results
US 12,644,140 B2Granted
Engineered microorganisms for synthesis of polyhydroxyalkanoates
OwnerTrevor Charles, Jiujun Cheng +2inventors
InventorsTrevor Charles, Jiujun Cheng, Aranksha Thakor, Nicole Schulz
Life of this patent
Estimating the term…
Abstract
A genetically engineered strain of Pseudomonas is provided, which is capable of metabolizing lactose as a sole carbon source in the production of polyhydroxyalkanoates (PHAs). A method of engineering microorganisms and a method of PHA production are also provided, wherein lactose is used as a sole carbon source.
Claims16
A cell of a microorganism, wherein the cell is genetically modified to synthesize polyhydroxyalkanoates, PHAs, using lactose as the sole carbon source, wherein:
- the microorganism is Pseudomonas alloputida KT2440;
- and the microorganism is genetically modified to incorporate in its genome non-native genes for lactose and galactose metabolization,
- wherein:
- the non-native genes are expressed in a single operon;
- the non-native genes for lactose metabolization are lacY and lacz obtained from Escherichia coli;
- and
- the non-native genes for galactose metabolization are galactose dehydrogenase (galD), 2-dehydro-3-deoxygalactonokinase (dgok), 2-dehydro-3-deoxy-6-phospho-galactonate aldolase (dgoA), and galactonate dehydratase (dgoD) obtained from Pseudomonas YsS1.
4 more elements
Dependencies are inferred from the claim text.