Compositions and methods related to megakaryocyte-derived extracellular vesicles for treating myeloproliferative neoplasms

A method for gene editing a cell comprising a mutation in JAK2 gene, the method comprising

• (a) contacting the cell with a composition comprising a plurality of substantially purified megakaryocyte-derived extracellular vesicles comprising a lipid bilayer membrane surrounding a lumen, wherein:
• the megakaryocyte-derived extracellular vesicle lumen comprises a cargo comprising an agent suitable for gene editing the cell and/or cargo comprising an agent suitable for gene editing the cell is associated with the surface of the megakaryocyte-derived extracellular vesicles;
• and
• the lipid bilayer membrane comprises two or more proteins associated with or embedded within, wherein the two proteins are CD31 and CD47, and
• (b) gene editing the cell to alter the mutation in the JAK2 gene therein.