Selective and high-resolution printing of single cells
AssigneeThe Regents of the University of California
InventorsAdam R. Abate, Pengfei Zhang, Russell Cole
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Abstract
Methods for on-demand printing discrete entities including, e.g., cells, media or reagents to substrates are provided. In certain aspects, the methods include manipulating qualities of the entities or biological components thereof. In some embodiments, the methods may be used to create arrays of microenvironments and/or for two and three-dimensional printing of tissues or structures and/or for in situ printing for microsurgeries. Systems and devices for practicing the subject methods are also provided.
Claims31
A method of delivering discrete entities to a substrate, the method comprising:
- flowing a plurality of discrete entities through an air flow via a microfluidic device comprising an air/liquid co-flow junction, wherein the air/liquid co-flow junction is connected to one or more air channels through which the air flow can be applied;
- directing the air flow and one or more of the plurality of discrete entities through the air/liquid co-flow junction to the substrate;
- and
- affixing the one or more of the plurality of discrete entities to the substrate via a force, wherein the force is selected from a gravitational force, an electrical force, a magnetic force, and combinations thereof.
Dependencies are inferred from the claim text.