Detection of environmental influences by bioluminescence
AssigneeRheinisch-Westfälische Technische Hochschule (RWTH) Aachen
InventorsJan Borlinghaus, Martin Gruhlke, Jana Foerster, Alan Slusarenko, Michael Eberhard Ries
Life of this patent
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Abstract
A nucleic acid comprising a continuous nucleotide sequence, containing: (i) a gene encoding LuxA, (ii) a gene encoding LuxB, (iii) a gene encoding LuxC, (iv) a gene encoding LuxD, (v) a gene encoding LuxE, wherein each of the genes is under the control of a promoter heterologous to the respective gene, and wherein all of the genes together with the promoter are contained in a single nucleotide sequence in a row. The vectors and host cells comprise the nucleic acid. Methods of producing a host cell and methods and uses for detecting an environmental effect and a kit therefor.
Claims17
A nucleic acid comprising a continuous nucleotide sequence containing:
- (i) a gene encoding LuxA,
- (ii) a gene encoding LuxB,
- (iii) a gene encoding LuxC,
- (iv) a gene encoding LuxD, and
- (v) a gene encoding LuxE,
- wherein each of the genes is under the control of a yeast, animal, viral or synthetic promoter heterologous to the respective gene, wherein all of the genes together with the promoter are contained in a single nucleotide sequence in a row, wherein the promoters mediate approximately equal expression levels, wherein each gene is followed by a terminator, and/or the nucleic acid does not contain an internal ribosomal entry site and/or self-cutting peptides between any of genes (i) to (vi).
A nucleic acid comprising a continuous nucleotide sequence containing:
- (i) a gene encoding LuxA,
- (ii) a gene encoding LuxB,
- (iii) a gene encoding LuxC,
- (iv) a gene encoding LuxD, and
- (v) a gene encoding LuxE,
- wherein each of the genes is under the control of a yeast promoter heterologous to the respective gene, wherein all of the genes together with the promoter are contained in a single nucleotide sequence in a row, wherein promoters mediate approximately equal expression levels, wherein each gene is followed by a terminator, and/or the nucleic acid does not contain an internal ribosomal entry site and/or self-cutting peptides between any of genes (i) to (vi).
Dependencies are inferred from the claim text.