Polypeptides capable of converting substrate 3-keto-deoxynivalenol into 3-epi-deoxynivalenol

A method of reducing the content of DON in a composition comprising DON or of reducing the toxicity of a composition comprising DON by converting DON into 3-epi-DON, the method comprising

• a) contacting the composition with an enzyme capable of converting DON into 3-keto DON;
• and
• b) subsequently or concurrently contacting the composition with one or more polypeptide(s) comprising or consisting of SEQ ID NO:
• 1, or a sequence having a sequence identity of at least at least 72.0% to SEQ ID NO:
• 1;
• wherein the polypeptide comprises one or more of the following polypeptide sequences:
• (i)
• (SEQ ID NO. 31)
• YRKLGNSG;
• (ii)
• (SEQ ID NO. 32)
• LGTMTFG;
• (iii)
• (SEQ ID NO. 33)
• AGGNFX1DTAX2VYS, wherein X1 is I or L and X2 is
• N or D;
• (iv)
• (SEQ ID NO. 34)
• ETLRFLDD;
• (v)
• (SEQ ID NO. 35)
• GKIX3YYGFSN, wherein X3 is A or G;
• (vi)
• (SEQ ID NO. 36)
• RDIEHEX4VPA, wherein X4 = I or V;
• (vii)
• (SEQ ID NO. 37)
• GLLPWSPLGGGWL;
• (viii)
• (SEQ ID NO. 38)
• GATRLGENP;
• (ix)
• (SEQ ID NO. 39)
• AQVALAW;
• and/or
• (x)
• (SEQ ID NO. 40)
• PAVX5SVILGART, wherein X5 = T or A.

A method of converting 3-keto-DON into 3-epi-DON, the method comprising contacting one or more polypeptide(s) with 3-keto DON, wherein the one or more polypeptide(s) comprise or consist of:

• (a) SEQ ID NO:
• 1, or (b) a sequence having a sequence identity of at least 72.0% to SEQ ID NO:
• 1, and at least 95.0% sequence identity to any one of SEQ ID NOs:
• 1-28.