Microbial cells, methods of producing the same, and uses thereof
AssigneesKansas State University Research Foundation, Axiota U.S., Inc.
InventorsJames Scott Drouillard, Celine Caroline Aperce, Gina Rae Herren, Tara Jo Ellerman, Ciana Marie Scaletti, Katherine Van Jordan, James Morris Lattimer, Scott Beyer, Solange Uwituze, Teresa Lea Douthit, Christina Denise Gunkel
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Abstract
The present invention relates to microbial cells, including but not limited to aerobic bacteria cells and anaerobic bacteria cells, as well as yeast cells, and methods for producing the cells, feed additives and compositions comprising the cells, and uses involving administration of the cells to animals.
Claims20
A method of producing freeze-dried anaerobic bacterial cells on a commercial scale, comprising:
- (a) preparing a liquid culture under anaerobic conditions or partial anaerobic conditions comprising anaerobic bacterial cells and a growth media comprising at least one carbon source,
- (b) harvesting the cells under anaerobic conditions or partial anaerobic conditions,
- (c) adding at least one cryoprotectant to the harvested cells,
- (d) freezing the cells,
- (e) freeze-drying the cells, and
- (f) obtaining about 1×107 to about 1×1012 CFU/g of viable anaerobic bacterial cells after freeze-drying, wherein the method is performed under anaerobic conditions on a commercial scale.
Dependencies are inferred from the claim text.