Method for culturing cells

A system comprising:

• a substrate comprising:
• a fluid channel system formed in the substrate, comprising:
• a culturing channel for growing a cell culture, comprising a culturing access to an outside;
• a storage channel for one or more selected from the group consisting of:
• storing, concentrating, homogenizing, and diluting a cell suspension containing transferred cells, comprising a storage access to the outside;
• and
• a transfer channel with a transfer access to the outside,
• wherein the culturing channel and the storage channel are fluidically connected to each other via the transfer channel;
• wherein at least one wall of the culturing channel, comprising at least a partial area of a floor and/or of side walls of the culturing channel, has a structuring which is configured in such a way that a growth surface is increased, the structuring comprising one or more selected from the group consisting of:
• a saw tooth profile, a wave profile, a lamellar structure, fibrous areas, and porous areas;
• wherein the substrate is configured to:
• receive an initial cell suspension through the culturing access into the culturing channel or through the transfer access via the transfer channel into the culturing channel;
• grow a cell culture in the culturing channel;
• transfer cells of the cell culture from the culturing channel into the storage channel via the transfer channel, wherein the transferring is to be carried out by supplying a transfer liquid through the culturing access, and transporting the transfer liquid through the culturing channel and the transfer channel into the storage channel;
• and
• enable one or more selected from the group consisting of:
• storing, concentrating, homogenizing, and diluting a cell suspension containing the transferred cells in the storage channel;
• at least three liquid containers arranged outside the substrate, wherein the culturing access, the storage access and the transfer access are each fluidically connected to at least one of the liquid containers;
• and
• active components, which are configured and arranged in such a way that, when actuated, they cause one or more selected from the group consisting of:
• a controlled supply of liquids from the liquid containers into the fluid channel system, or a controlled transport of liquids in the fluid channel system, and a controlled discharge of liquids from the fluid channel system, wherein the active components are arranged outside the substrate.