Back to results
US 12,644,086 B2Granted
Rolled scaffold for large scale cell culture in monolayer
AssigneeBoard of Supervisors of Louisiana State University and Agricultural and Mechanical College
InventorKidong Park
Life of this patent
Estimating the term…
Abstract
The present invention provides rolled scaffold devices and cell culture systems that can provide a large surface-area-to-volume ratio for expanded cell culture. The rolled scaffolds minimize shear stress on cultured cells and support sufficient and uniform mass transfer rates of gases and nutrients. The rolled scaffolds can be connected to a media source via holders to support large-scale expansion and maintenance of cell cultures.
Claims6
A rolled scaffold comprising:
- a planar film having an upper surface, a lower surface, a length, and a width;
- a plurality of elongate spacers attached to one of the surfaces, the spacers having a height, a width, and a length that spans the entire width of the film;
- wherein the film is rollable along its length into a cylindrical shape such that the plurality of elongate spacers face and press against the opposing surface of the rolled film, and wherein the height of the plurality of elongate spacers maintain a space between the rolled film layers, wherein the plurality of elongate spacers are set apart at intervals to form discrete channels;
- and
- a casing having a hollow interior sized to encase the rolled scaffold, wherein the casing is formed from a first and a second endcap connectable at a junction, wherein the first endcap and the second endcap respectively comprise a first tapered opening and a second tapered opening, wherein the first and second tapered openings are in fluid communication with the hollow interior and are capable of passing at least one fluid through the rolled scaffold.
2 more elements
A rolled scaffold comprising:
- a planar film having an upper surface, a lower surface, a length, and a width;
- a plurality of elongate spacers attached to one of the surfaces, wherein the plurality of elongate spacers have a height, a width, and a length that spans the entire width of the film, wherein the film is rollable along its length into a cylindrical shape such that the plurality of elongate spacers face and press against the opposing surface of the rolled film, wherein the height of the plurality of elongate spacers maintain a space between the rolled film layers, wherein the plurality of elongate spacers are set apart at intervals to form discrete channels;
- and
- a casing having a hollow interior sized to encase the rolled scaffold, wherein the casing is formed from a first and a second endcap connectable at a junction, wherein the first endcap and the second endcap respectively comprise a first tapered opening and a second tapered opening, wherein the first and second opening are aligned along a longitudinal central axis of the rolled scaffold, wherein the first and second tapered opening are in fluid communication with the hollow interior and are capable of passing at least one fluid directly along the path of the longitudinal central axis and through the rolled scaffold.
1 more element
A rolled scaffold comprising:
- a planar film having an upper surface, a lower surface, a length, and a width;
- a plurality of elongate spacers attached to one of the surfaces, wherein the plurality of elongate spacers have a height, a width, and a length that spans the entire width of the film, wherein the plurality of elongate spacers comprise ultraviolet curing material, wherein the plurality of elongate spacers are cured with ultraviolet light onto the planar film, wherein the film is rollable along its length into a cylindrical shape such that the plurality of elongate spacers face and press against the opposing surface of the rolled film, wherein the height of the plurality of elongate spacers maintain a space between the rolled film layers, wherein the plurality of elongate spacers are set apart at intervals to form discrete channels;
- and
- a casing having a hollow interior sized to encase the rolled scaffold, wherein the casing is formed from a first and a second endcap connectable at a junction, wherein the first endcap and the second endcap respectively comprise a first tapered opening and a second tapered opening, wherein the first and second opening are aligned along a longitudinal central axis of the rolled scaffold, wherein the first and second tapered opening are in fluid communication with the hollow interior and are capable of passing at least one fluid directly along the path of the longitudinal central axis and through the rolled scaffold.
1 more element
Dependencies are inferred from the claim text.