Dual circulation microphysiological system

A microphysiological cell culture system, the system comprising:

• a cell culture chamber;
• first and second fluidic circuits, each connected to said chamber at a first and second fluidic circuit chamber outlet and a chamber inlet, wherein at least the first and second circuit chamber outlets are at separate positions;
• wherein the cell culture chamber defines a first fluid fill level and a second fluid fill level, the first fluid fill level representing a greater volume of fluid than the second fluid fill level;
• and
• wherein the first fluidic circuit chamber outlet is positioned at the first fluid fill level or between the first and second fluid fill levels, and the second fluidic circuit chamber outlet is positioned at or below the second fluid fill level;
• such that either the first or both first and second fluidic circuits are able to operate when the chamber is filled with fluid to the first fill level, and only the second fluidic circuit is able to operate when the chamber is filled with fluid to the second fill level.