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US 12,643,928 B2Granted
Split intein mediated protein polymerization for microbial production of materials
AssigneeWashington University
InventorsFuzhong Zhang, Christopher H. Bowen, Bin Dai
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Abstract
The present disclosure is directed to systems and methods for synthesizing a spidroin. In some embodiments, the methods comprise synthesizing a monomer in vivo in a heterologous host, the monomer comprising an N-terminus IntC domain and a C-terminus IntN domain, and post-translationally polymerizing the synthesized monomer via in vitro split-intein mediated polymerization.
Claims15
A system for synthesizing a spidroin in vivo, the system comprising:
- a heterologous host cell;
- a seed cassette encoding a seed protein comprising a C-terminus IntN domain;
- and
- a monomer cassette encoding a monomer comprising an N-terminus IntC domain and a C-terminus IntN domain, wherein the monomer is a spidroin fragment, and wherein the monomer cassette encodes a monomer comprising an N-terminus IntC domain comprising the amino acid sequence of SEQ ID NO:
- 10 and a C-terminus IntN domain comprising the amino acid sequence of SEQ ID NO:
- 11.
3 more elements
Dependencies are inferred from the claim text.