Method of clarifying a crude protein solution
AssigneeCytiva BioProcess R&D AB
InventorGustav Rodrigo
Life of this patent
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Abstract
Method of clarifying a crude protein solution, the method comprising to provide (100) a volume of the crude protein solution, mixing (101) Na2HPO4 and CaCl2) in water, forming a first solution, adding (102) the first solution to the volume of crude protein solution, thereby forming a second solution. NaCl is added to the first solution, the crude protein solution and/or to the second solution. The second solution is mixed (103). The thus formed flocculated material is separated (104) from the second solution, obtaining a clarified protein solution. The clarified protein solution may thereafter be purified by chromatography.
Claims7
Method of clarifying a crude protein solution, the method comprising:
- providing a volume of the crude protein solution;
- mixing Na2HPO4 and CaCl2 in water, forming a first solution;
- adding the first solution to the volume of crude protein solution, thereby forming a second solution;
- wherein NaCl is added to the first solution, the crude protein solution and/or to the second solution;
- mixing the second solution;
- separating thus formed flocculated material from the second solution, obtaining a clarified protein solution.
Dependencies are inferred from the claim text.