Probe related reagents and methods
AssigneeBrentano Biotechnology Associates
InventorKenneth A. Browne
Life of this patent
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Abstract
A versatile reagent with a non-nucleotide monomeric unit comprising an enantiomeric center and having a ligand, and first and second coupling groups that are linked to the non-nucleotide monomeric unit. Such a reagent permits preparation of versatile nucleotide/non-nucleotide polymers, having any desired sequence of nucleotide and non-nucleotide monomeric units, each of the latter of which bear a desired ligand. These polymers can, for example, be used as probes which can exhibit enhanced sensitivity and/or which are capable of detecting a genus of nucleotides each species of which has a common target nucleotide sequence of interest bridged by different sequences not of interest.
Claims4
A method of manufacturing a polymer incorporating an enantiomeric linking compound comprising the steps of:
- (a) first coupling a reactive phosphorus group of the enantiomeric linking compound to a nucleic acid monomer or a nucleic acid polymer under polymer synthesis conditions;
- (b) removing a dimethoxytrityl protecting group from the enantiomeric linking compound to permit a second coupling of another nucleic acid monomer, another nucleic acid polymer, or another enantiomeric linking compound to the enantiomeric linking compound;
- and
- (c) removing a fluorenyimethyloxycarbonyl protecting group from the enantiomeric linking compound to permit a third coupling of a luminescent moiety,
- wherein the enantiomeric linking compound is composed of a plurality of units having a single enantiomeric form, wherein each unit of the plurality of units is:
- wherein n is 1, 2, 4, or 5, or
- wherein the plurality of units are synthesized with(S)-3-amino-1,2-propanediol, or wherein each unit of the plurality of units is:
- wherein n is 1, 2, 4, or 5, or
- wherein the plurality of units are synthesized with (R)-3-amino-1,2-propanediol.
A method of manufacturing a polymer incorporating an enantiomeric linking compound comprising the steps of:
- (a) first coupling a reactive phosphorus group of the enantiomeric linking compound to a nucleic acid monomer or a nucleic acid polymer under polymer synthesis conditions;
- (b) removing a dimethoxytrityl protecting group from the enantiomeric linking compound to permit a second coupling of another nucleic acid monomer, another nucleic acid polymer, or another enantiomeric linking compound to be coupled to the enantiomeric linking compound;
- and;
- (c) removing a fluorenylmethyloxycarbonyl protecting group from the enantiomeric linking compound to permit a third coupling of a luminescent moiety,
- wherein the enantiomeric linking compound is composed of a plurality of units having a single enantiomeric form, wherein each unit of the plurality of units is:
- wherein the units are synthesized with(S)-4-aminobutane-1,3 diol;
- or
- wherein each unit of the plurality of units is:
- wherein the units are synthesized with (R)-4-aminobutane-1,3 diol.
Dependencies are inferred from the claim text.