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US 12,643,868 B2Granted
Reagent for mass spectrometry
AssigneeRoche Diagnostics Operations, Inc.
InventorsDieter Heindl, Hans-Peter Josel, Uwe Kobold, Christoph Seidel, Martin Rempt, Andreas Leinenbach, Giuseppe Prencipe, Silvia Baecher, Simon Ferdinand Loibl, Anna-Skrollan Geiermann, Jelena Milic, Nicole Pirkl
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Abstract
The present invention relates to reagents which are suitable to be used in mass spectrometry as well as methods of mass spectrometric determination of analyte molecules using said reagents.
Claims9
A method for the mass spectrometric determination of an analyte molecule comprising the steps:
- (a) reacting the analyte molecule with a compound of formula A
- X-L1-Y-L2-Z
- wherein
- X is a reactive unit, which is capable of forming a covalent bond with an analyte molecule,
- L1 and L2 are independently of each other substituted or unsubstituted linker,
- Y is a neutral loss unit,
- Z is a charged unit comprising at least one permanently charged moiety, wherein the reactive unit X is H2N—NH—, the neutral loss unit Y is triazole or tetrazole, and the charge unit Z comprises a piperidine unit, or wherein the compound is selected from the group consisting of:
- whereby a covalent adduct of the analyte molecule and the compound of formula A is formed by the chemical reaction of the reactive unit with the analyte molecule, and
- (b) subjecting the adduct from step (a) to a mass spectrometric analysis.
6 more elements
Dependencies are inferred from the claim text.