Modified bacterial strains for improved fixation of nitrogen
AssigneePivot Bio, Inc.
InventorsKarsten Temme, Neal Shah, Bilge Ozaydin Eskiyenenturk, Sarah Bloch, Alvin Tamsir
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Abstract
Methods and systems are provided for generating and utilizing a genetically engineered bacterium comprising a modification in a gene regulating nitrogen fixation or assimilation, wherein the modification in the gene regulating nitrogen fixation or assimilation results in one or more of: constitutive expression of a nifA gene in nitrogen limiting and non-nitrogen limiting conditions, activity of nifA in non-nitrogen limiting conditions, decreased uridylyl-transferase activity of GlnD, decreased adenylyl-removing activity of GlnE, and increased ammonium excretion.
Claims14
A method of increasing the amount of atmospheric derived nitrogen in a plant, the method comprising contacting the plant, a part of the plant, or soil into which the plant is planted with a plurality of genetically engineered Klebsiella variicola bacteria comprising one or more genetic modifications in a gene regulating nitrogen fixation or assimilation and a plurality of genetically engineered Kosakonia sacchari bacteria comprising one or more genetic modifications in a gene regulating nitrogen fixation or assimilation, wherein:
- the one or more genetic modifications in the plurality of genetically engineered Klebsiella variicola bacteria comprise a deletion of all or a portion of the coding sequence of the nifL gene and a deletion of a portion of the coding sequence of the glnD gene, and
- the one or more genetic modifications in the plurality of genetically engineered Kosakonia sacchari bacteria comprise a deletion of all or a portion of the coding sequence of the nif gene, a deletion of all of the coding sequence of the glnD gene, and a deletion of a portion of the coding sequence of the glnE gene.
A composition comprising a genetically engineered Klebsiella variicola bacterium and a genetically engineered Kosakonia sacchari bacterium, wherein the genetically engineered Klebsiella variicola bacterium and the genetically engineered Kosakonia sacchari bacterium each comprise one or more genetic modifications in a gene regulating nitrogen fixation or assimilation, wherein:
- the one or more genetic modifications in the genetically engineered Klebsiella variicola bacterium comprise a deletion of all or a portion of the coding sequence of the nifL gene and a deletion of a portion of the coding sequence of the glnD gene, and
- the one or more genetic modifications in the genetically engineered Kosakonia sacchari bacterium comprise a deletion of all or a portion of the coding sequence of the nifL gene, a deletion of all of the coding sequence of the glnD gene, and a deletion of a portion of the coding sequence of the glnE gene.
A microbial composition comprising a Kosakonia sacchari bacterium represented by a bacterium deposited as ATCC Accession No. PTA-126743, and a Klebsiella variicola bacterium represented by a bacterium deposited as ATCC Accession No. PTA-126740.
Dependencies are inferred from the claim text.