Regulatable adeno-associated virus (AAV) vector

An AAV vector construct comprising

• (i) a first expression cassette comprising a first promoter and a coding sequence for a regulator protein, wherein the first promoter directs expression of the regulator protein under the control of a first promoter, wherein the regulator protein is activated in the presence of an activator molecule, and wherein the regulator protein comprises a GAL4 DNA binding domain, a progesterone receptor ligand binding domain, and the p65 transactivation domain from NF-kappaB, and
• (ii) a second expression cassette comprising a promoter region and a multiple cloning site allowing the insertion of a coding sequence for a molecule of interest, wherein the promoter region regulates expression of the molecule of interest following insertion of the coding sequence for the molecule of interest, wherein the promoter region comprises one or more GAL4 binding sites and a second promoter, and wherein the expression of the molecule of interest is induced by binding of the activated regulator protein to the promoter region, wherein the first expression cassette and the second expression cassette are arranged in a tail-to-head configuration, and wherein the first expression cassette and the second expression cassette are separated by a transcription blocker sequence comprising a transcription pause site and a polyadenylation signal sequence.