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US 12,642,820 B2Granted
Multistrain population control systems and methods
AssigneeThe Regents of the University of California
InventorsJeff Hasty, Michael Julius Liao, Muhammad Omar Din
Life of this patent
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Abstract
Provided herein are multi-strain population control systems, methods, kits, and compositions. Also provided are methods, systems, kits, and compositions for culturing bacterial cells in multi-strain ecosystems, and temporally arranged multi-strain ecosystems or cultures using a synchronized lysis circuit in combination with multiple toxin/antitoxin systems to cycle continuously over a long period of time.
Claims12
A method of delivering a therapeutic agent to a subject, comprising:
- administering to the subject a therapeutically effective amount of each of n bacterial strains comprising at least a first bacterial strain, a second bacterial strain, and an nth bacterial strain that are distinct from each other;
- wherein n is at least 3;
- wherein each of the n bacterial strains comprises a toxin system;
- wherein the toxin system of the first bacterial strain produces a first toxin/first antitoxin pair and an nth antitoxin from an nth toxin/nth antitoxin pair, wherein the first toxin is effective against the nth bacterial strain;
- wherein the toxin system of the second bacterial strain produces a second toxin/second antitoxin pair and the first antitoxin from the first toxin/first antitoxin pair,
- wherein the second toxin is effective against the first bacterial strain;
- and
- wherein the toxin system of the first bacterial strain produces an nth toxin/nth antitoxin pair and an (n−1)th antitoxin from an (n−1)th toxin/(n−1)th antitoxin pair, wherein the nth toxin is effective against the (n−1)th bacterial strain;
- and
- wherein one or more of the n bacterial strains express a therapeutic agent.
7 more elements
Dependencies are inferred from the claim text.