Use of plant exosomes for enhancing viability and hair growth capacity of skin cells

A method of enhancing viability of skin cells and enhancing hair growth by stimulating hair follicles, comprising administering a composition of plant exosomes to skin cells, wherein administering the composition of plant exosomes induces a transforming growth factor beta 1 (TGF-B1) gene expression, a Wnt family member 5A (WNT5a) gene expression, and a Sonic Hedgehog (Shh) gene expression in the skin cells, and the plant exosomes are isolated from a pineapple plant, a garlic plant, and a pomegranate plant, wherein

• the administered doses per 100 μL are 1 ng-1 mg of the plant exosomes isolated from the pineapple plant, 1 ng-10 mg of the plant exosomes isolated from the garlic plant, and 1 ng-1 mg of the plant exosomes isolated from the pomegranate plant, and wherein
• the plant exosomes are isolated from a plant lysate by an isolation method, comprising:
• removing large size particles and impurities resulting from plant disintegration by centrifugation performed between 2,000 g and 10,000 g for 5-20 minutes,
• filtering to remove particles sized 220 nanometers and above, and
• separating an obtained homogeneous exosome-protein mixture by subjecting the mixture to a two-phase liquid system comprising polyethlene glycol (PEG) and dextran (DEX), and collecting the DEX phase to obtain the plant exosomes, wherein the plant exosomes are cleared of nonexosomal proteins, cellular fats, and other impurities.