Method and assembly for in-ovo sexing of bird eggs

A method for in-ovo sexing of bird eggs, wherein the following method steps are carried out:

• providing a fertilized bird egg incubated for between 3 and 9 days,
• producing an opening in the shell of the bird egg in the region of the sharp or blunt pole,
• irradiating and exciting blood of at least one constituent of the formed extraembryonic cardiovascular system of the embryo using at least two light-emitting laser beam sources which have different excitation wavelengths λ in the range of 400 nm to 1064 nm and simultaneously excite fluorescence scattering and Raman scattering,
• capturing the spectra of the fluorescence scattering and Raman scattering, the spectra being excited in the blood and separated using at least two beam splitter devices and according to wavelength ranges, by means of at least two detector devices and processing the spectra by means of an evaluation unit,
• outputting the captured and processed spectra of the fluorescence scattering and Raman scattering as complementary spectra assigned to the different excitation wavelengths,
• ascertaining a classification value using the output complementary spectra of a selected spectral range and/or spectral intensity maxima ISmax of the fluorescence scattering and Raman scattering assigned to the respective excitation wavelengths,
• determining the sex by comparing the ascertained classification value with an intrinsically ascertained threshold value.