Dual magnification fluorescence imaging camera

A video camera device for white light and fluorescence imaging comprising:

• a first optical subsystem that transmits reflected and scattered light for a non-fluorescent image and transmits emitted fluorescence light for a fluorescence image, both fluorescence and non-fluorescence light collected from an illuminated scene;
• a beamsplitter downstream from the first optical subsystem which separates the light for said non-fluorescence image into a first imaging channel for and light for said fluorescence image into a second imaging channel;
• a meniscus lens positioned downstream from the beamsplitter in one of the first and second imaging channels, the meniscus lens functional to change a magnification such that a magnification of the first imaging channel is different from a magnification of the second imaging channel;
• and
• a single focal plane array image sensor positioned downstream of the beamsplitter to receive the fluorescence and non-florescence light;
• wherein the image sensor is configured to image the non-fluorescence image light from the first imaging channel onto a first location of the image sensor and image the fluorescence image light from the second imaging channel onto a second image location on the image sensor, wherein the first location on the image sensor is distinct from and non-overlapping with the second location on the image sensor, as, respectively, the non-fluorescence image and the fluorescence image, and wherein the fluorescence image has an image size with a smaller area on the sensor than an image size of the non-fluorescence image.