Stable reference materials for automated hematology testing platforms

A hematology reference material (HRM) comprising a suspension fluid and:

• a red blood cell (RBC) component that comprises human erythrocytes fixed with a crosslinking agent and hydrogel particles substantially similar to human red blood cells containing hemoglobin;
• a platelet component that comprises goat erythrocytes fixed with a crosslinking agent and hydrogel particles substantially similar to human platelets;
• a reticulocyte component that comprises fixed human erythrocytes encapsulated with RNA and hydrogel particles substantially similar to human reticulocytes;
• a lymphocyte component that comprises chicken erythrocytes fixed with a crosslinking agent and hydrogel particles substantially similar to human lymphocytes;
• a monocyte component that comprises turkey erythrocytes fixed with a crosslinking agent and hydrogel particles substantially similar to human monocytes;
• a basophil component that comprises alligator erythrocytes fixed with a crosslinking agent and hydrogel particles substantially similar to human basophils;
• a neutrophil component that comprises alligator erythrocytes fixed with a crosslinking agent and hydrogel particles substantially similar to human neutrophils;
• an eosinophil component that comprises alligator erythrocytes fixed with a crosslinking agent and hydrogel particles substantially similar to human eosinophils;
• an immature reticulocyte component that comprises fixed human erythrocytes encapsulated with RNA and hydrogel particles substantially similar to immature human reticulocytes;
• an immature granulocyte component that comprises alligator erythrocytes fixed with a crosslinking agent and hydrogel particles substantially similar to immature human granulocytes;
• an immature thrombocyte component that comprises goat erythrocytes fixed with a crosslinking agent and hydrogel particles substantially similar immature human thrombocytes;
• and
• a nucleated red blood cell component that comprises chicken erythrocytes fixed a crosslinking agent and hydrogel particles substantially similar to nucleated human red blood cells,
• wherein each of said components exhibit a forward scatter profile or a side scatter profile that is at least 95% similar to its corresponding component in a human blood sample.