Back to results
US 12,638,440 B2Granted
Organ models
AssigneeThe Penn State Research Foundation
InventorsIbrahim Tarik Ozbolat, Monika Hospodiuk, Dino Joseph Ravnic, Bugra Ayan, Srinivas Koduru
Life of this patent
Estimating the term…
Abstract
This document provides materials and methods for making and using functional (e.g., vascularized) organ models (e.g., pancreas models). For example, functional pancreas models including an ECM containing a plurality (e.g., two or more) of pancreatic islets, and a vascular network are provided.
Claims20
A method of making a pancreas model, said method comprising:
- disposing a first layer of extracellular matrix onto a substrate assembled into a model platform;
- disposing a strand of sacrificial matrix onto an interior region of said first layer of extracellular matrix;
- using an aspiration-assisted bioprinting process to bioprint an array of engineered pancreatic islets comprising pre-vascularized pancreatic spheroids onto said first layer of extracellular matrix;
- disposing a second layer of extracellular matrix onto said array of pancreatic islets;
- removing said sacrificial matrix with an aspiration removal process to create a channel;
- seeding said channel with microvessel endothelial cells;
- and
- perfusing said channel with perfusate,
- wherein said pancreas model comprises from about 20 islets to about 50 islets per mm3 of said pancreas model, and
- wherein said pancreas model comprises a vascular network comprising microvascularization within pancreatic islets.
7 more elements
A method of making a pancreas model, said method comprising:
- disposing a first layer of extracellular matrix onto a substrate assembled into a model platform;
- disposing a strand of sacrificial matrix onto an interior region of said first layer of extracellular matrix;
- disposing an array of engineered pancreatic islets comprising pre-vascularized pancreatic spheroids onto said first layer of extracellular matrix;
- disposing a second layer of extracellular matrix onto said array of pancreatic islets;
- removing said sacrificial matrix with a solution to create a channel;
- seeding said channel with microvessel endothelial cells;
- and
- perfusing said channel with perfusate,
- wherein said pre-vascularized pancreatic spheroids are engineered, prior to disposing said first layer of extracellular matrix onto said substrate, by co-culturing beta cells and microvessel endothelial cells,
- wherein said beta cells and said microvessel endothelial cells are co-cultured in a ratio of from about 1:1 to about 10:1,
- wherein said pancreas model comprises from about 20 islets to about 50 islets per mm3 of said pancreas model, and
- wherein said pancreas model comprises a vascular network comprising microvascularization within pancreatic islets.
9 more elements
Dependencies are inferred from the claim text.