Back to results
US 12,638,439 B2Granted
Method of using human spheroids for drug discovery
AssigneeAXOSIM, Inc.
InventorsCassiano Carromeu, Robert T. Fremeau, Jr.
Life of this patent
Estimating the term…
Abstract
The present invention discloses, in one embodiment, a method of using human induced pluripotent stem cells to generate three-dimensional human organ tissue for therapeutic drug toxicity and discovery⋅. In one embodiment, a high throughput microtiter plate is loaded with both wild type and Rett disease 3D spheroids and exposed to a drug library, and activity is measured and analyzed for disease rescue to wild type cell behavior.
Claims20
A method to detect the effect of one or more compounds on reversing or rescuing erratic calcium oscillation activity in spheroids, comprising:
- contacting one or more spheroids of human cells of uniform diameter and one or more test compounds, wherein the spheroids are obtained from cells of an autism patient or cells of a Rett syndrome patient;
- and
- detecting the effect on reversing or rescuing erratic calcium oscillation activity of the one or more compounds on one or more of the spheroids.
Dependencies are inferred from the claim text.